Pulsed field gel electrophoresis protocols methods and theories methods in molecular biology softcover reprint of the original 1st ed 1992 edition. Pulsed homogeneous orthogonal field gel electrophoresis phoge the difference among this method and other methods of pfge with homogeneous fields is the orientation angle of 90 degrees in field dna molecules are moved four times per cycle instead of twice. Pulsed field gel electrophoresis pulse electroo phoresis pfge is one of the methods widely used in molecular biology to separate large dna fragg ments from a few hundreds of kilobase pairs . This protocol describes pulsed field gel electrophoresis pfge a method developed for separation of large dna molecules whereas standard dna gel electrophoresis commonly resolves fragments up
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